Name

2x Genotyping PCR Ready Master Mix (250 x 20µL reactions)

Cat. #

Application

Genotyping PCR

DNA mutagenesis

The 2x Genotyping PCR Ready Mix is a ready-to-use solution containing all reagents required for PCR, including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, tracking dye, density, PCR stabilizer and enhancer at optimal concentrations for consistent and efficient PCR amplification. It simplifies PCR assembly, so that only primers, DNA template and water need to be added, and the PCR products can be directly loaded onto electrophoresis gel(See image).

Convenient: 2x Genotyping PCR Ready Mix contains all components for PCR amplification. All you need to do is to add primers, template, and water for PCR reaction.

No Loading Dye required: The PCR products can be directly loaded onto electrophoresis gel.

High efficiency: the optimal buffer condition and specific engineered Taq DNA polymerase have increased the efficiency of PCR amplifications.

Shipping / Storage

Manual (protocol)

Suggested PCR Protocol

20 mL/reaction

• 2x PCR Mix: 10 mL
• Forward Primer: 10-20 pmol
• Reverse Primer: 10-20 pmol
• DNA template: <2 mL
• Nuclease-free H2O: up to 20 ml

Suggested PCR Conditions

• Step 1: 95°C for 5-10 minutes
• Step 2: 95°C for 10-30 seconds
• Step 3: 55-65°C for 30-90 seconds
• Step 4: 72°C for 30-120 seconds
• Step 5: 25-35 cycles of steps 2-4
• Step 6: 72°C for 5-10 minutes