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101Bio P107 PureExo® Exosome Isolation kit (for stem cell culture media), 10rxn


Supplier: 101Bio.com

Price: SGD$851.54

PureExo® Exosome Isolation kit (for stem cell culture media)

Why our exosome so pure?
Answer: By targeted filtration, our filter of specific binding treatment removes protein contaminant to isolate exosome at 95% high purity.        Click to see pure exosome images

Name

PureExo® Exosome Isolation Kit (for stem cell culture media)

Cat. #

 P107S
(  2 reactions)

P107

Application

Isolate pure exosome from stem cell culture media (supernatant). This kit yields much higher purity than exosome precipitation method. This product is for research use only.

Yield

 Each reaction can process 20 mL stem cell culture medium. The yield of each reaction can yield pure exosome suspended in 50 ~ 200 µL PBS (from which 100 ~ 1000 µg exosomal protein or 50 ~ 300 ng exosomal RNA can be extracted using our Exosomal RNA and Protein Extraction Kit, Cat.#: P200).

Description

P107 kit is for exosome purification / isolation at high yield and purity from stem cell culture media.

  • Easy to use: No ultra-centrifugation (< 2="">
  • 10 fold higher yield (vs. other kits and ultracentrifuge)
  • Save cost (vs. antibodies-beads method)
  • Isolate Pure exosome (>95% purity vs. 25% purity by exosome precipitation method)
  • Intact exosome (good morphology)

This kit isolate high yield pure exosomes for any downstream applications: EM study, exosome label, exosome subpopulation, qRT-PCR profiling of exosomal miRNAs, and gel analysis of exosomal proteins.

Shipping / Storage

 Ship and store at room temperature. Upon receiving keep all bottles upright, in cool and dark place.

Shelf Life

 12 months

Manual (protocol)

     Exosome Isolation Kit

Data Sheet

Components
ComponentsAmountStorage
Cat.#: P107S Cat.#: P107  
Solution A (blue) 1.5 mL 7.5 mL Room temperature
Solution B 1.5 mL 7.5 mL Room temperature
Solution C 6 mL 10 mL x 3 bottles Room temperature
PureExo&reg Column 2 10 Room temperature

Remarks

 The maximum medium volume of each reaction is 20 mL from at most 1 x 107 cells. Do not exceed the suggested sample volume or the cell number. Otherwise it may cause indistinct layer separation and column clogging. One PureExo® Column is only for one reaction.