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101Bio P130 Exosome-TEM-easy Kit, 10rxn


Supplier: 101Bio.com

Price: SGD$828.46

Exosome-TEM-easy Kit 

Name

Exosome-TEM-easy Kit (prepare exosome for transmission electron microscopy imaging)

Cat. #

 P130
Exosome   vs   Endosome Isolation Kit

Application

 This kit is for preparing exosome samples for transmission electron microscopy imaging assay.      This product is for research use only.

Product Size

 10 reactions

Description

 This is an easy-to-use tool helps you to prepare exosome samples for transmission electron microscope imaging, and to get good quality EM images of exosome structure.  This kit contains all reagents and material for the experiment.   The exosome purity and density is important for TEM assay.  We recommend to use our PureExo kits to isolate exosomes before using this kit.

Shipping / Storage

 Ship and store at room temperature

Shelf Life

 12 months

Manual (protocol)

     Exosome-TEM-easy Kit

Citation
Citation 1:
Heedoo Lee, Duo Zhang, Ziwen Zhu , Charles S. Dela Cruz, Yang Jin.   Epithelial cell-derived microvesicles activate macrophages and promote inflammation via microvesicle-containing microRNAs.  Sci Rep. 2016 Oct 12;6:35250. doi: 10.1038/srep35250.
Citation 2:
Abu Musa Md Talimur Reza, Yun-Jung Choi, Hideyo Yasuda, and Jin-Hoi Kim.   Human adipose mesenchymal stem cell-derived exosomal-miRNAs are critical factors for inducing anti-proliferation signalling to A2780 and SKOV-3 ovarian cancer cells.  Sci Rep. 2016 Dec 8;6:38498. doi: 10.1038/srep38498.
Citation 3:
Duo Zhang, Heedoo Lee, Ziwen Zhu, Jasleen K Minhas and Yang Jin.   Enrichment of selective miRNAs in exosomes and delivery of exosomal miRNAs in vitro and in vivo.  Am J Physiol Lung Cell Mol Physiol. 2017 Jan 1;312(1):L110-L121. doi: 10.1152/ajplung.00423.2016. Epub 2016 Nov 23.
 

Components
ComponentsAmountStorage
Formvar-carbon coated EM mesh 400 grid 10 room temperature
Wash buffer 1.5 mL room temperature
EM solution 0.1 mL room temperature

Other required materials
  • Parafilm
  • Forceps (Dumont No. 5), clean
  • Whatman Grade 1   Qualitative Filter Paper
  • Transmission electron microscope (TEM)
    Transimission Electron Microscopy image by our kit