P200R Exosomal RNA and Protein Extraction kit (Cat.#: P200, P200R, P200P), 20 run

101Bio P200R Exosomal RNA and Protein Extraction kit (Cat.#: P200, P200R, P200P), 20 run

Supplier: 101Bio.com

Price: SGD$853.00

Quantity:

Exosomal RNA and Protein Extraction kit (Cat.#: P200, P200R, P200P)

Name

Exosomal RNA and Protein Extraction Kit (Cat.# P200)

Cat. #

P200

P200R

(Exosomal RNA Extraction Kit only)

P200P

(Exosomal Protein Lysis Buffer only)

Exosomal DNA Extracation Kit (cat.# P230)

Exosome vs Endosome Isolation Kit (Cat.# P528)

Application

For extracting both exosomal RNA (including microRNA) and/or exosomal protein from pure exosome isolated by our Exosome Isolation Kits (Cat.#: P100, P101, P120, P121). This product is for research use only.

Product Size

20 reactions

Yield

In each reaction, from 50 µL ~ 100 µL pure exosome, 50 ~ 100 ng exosomal RNA or 150 ~ 200 µg exosomal protein can be extracted.

Shipping / Storage

Ship at 4℃, and store at -20℃ and room temperature (see component stable below). Upon receiving keep all bottles upright, at proper temperature (component table below) in dark place.

Shelf Life

6 months

Manual (protocol)

Cat.# P200 Exosomal RNA and Protein Extraction Kit

Cat.# P200R Exosomal RNA Extraction Kit

Cat.# P200P Exosomal Protein Lysis Buffer

Citation

Citation 1:

Li X, Liu L, Yang J, Yu Y, Chai J, Wang L, Ma L, Yin H. Exosome Derived From Human Umbilical Cord Mesenchymal Stem Cell Mediates MiR-181c Attenuating Burn-induced Excessive Inflammation. EBioMedicine. 2016, http://dx.doi.org/10.1016/j.ebiom.2016.04.030

Components

Components (Cat.# P200R)	Amount	Storage
N1	5 mL	Room temperature
N2	1 mL	Room temperature
N3	2.5 mL	Room temperature
N4	10 mL	Room temperature
RNA Elution Buffer	0.5 mL	Room temperature

Components (Cat.# P200P)	Amount	Storage
Exosomal Protein Lysis Buffer *	2 mL	- 20℃

* Store at 4℃ for 7 days or aliquot and store at - 20℃ for up to 6 months.

Remarks

Important: RNA is sensitive to RNase. Before starting RNA extraction, prepare clean lab bench and wipe working surface and pipettors with RNase decontamination solution, such as Ambion^® RNaseZap^®. Always wear clean laboratory gloves during manipulation.