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101Bio P201 Detergent-free Exosomal Protein Extraction Kit, 10 run
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Detergent-free Exosomal Protein Extraction Kit for proteomics (LC/MS)
Name
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Detergent-free Exosomal Protein Extraction Kit for proteomics (LC/MS)
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Cat. #
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P201
P201S
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Application
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For extracting detergent-free exosomal protein from pure exosome isolated by our Exosome Isolation Kits (Cat.#: P100, P101, P120, P121). This product is for research use only. |
Product Size
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P201: 10 reactions; P201S: 2 reactions |
Yield
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In each reaction, 150 ~ 200 µg exosomal protein can be extracted from 10 µl ~ 50 µL pure exosome. |
Description
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This kit is for rapidly extract detergent-free total proteins from exosome in less than 5 minute. The protein extraction buffers do not contain any detergent and EDTA. The whole procedure is less than 5 minutes.
The extracted protein can be used for proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. |
Shipping / Storage
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Ship room temperature and store at 4℃. |
Shelf Life
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12 months |
Manual (protocol)
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Detergent-free Exosomal Protein Extraction Kit |
Components
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| Components | P201 | P201S | Storage |
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| Buffer A |
500 µl |
100 µl |
4℃ |
| Buffer B |
500 µl |
100 µl |
4℃ |
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Remarks
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The use of protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, addition of protease inhibitors to Buffer A is recommended. For determination of protein concentration, BCA kit is recommended. To study protein phosphorylation, phosphatase inhibitors (such as PhosStop from Roche) should be added to Buffer A prior to use. |
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