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101Bio P400 ExoFectin® sRNA-into-Exosome Kit (Electro), 10 rxn


Supplier: 101Bio.com

Price: SGD$1,105.38

ExoFectin® sRNA-into-Exosome Kit  (Electro) 

Name

ExoFectin® sRNA-into-Exosome Kit  (Electro)

Cat. #

 P400
(10 reactions)

Application

 Load nuclei acids ( miRNA and siRNA ) into pure exosomes ( isolated by our kits: Cat.# P100, P101, P120, P121 ).  This product is for research use only.

Yield

 50% ~ 85% transfection efficiency

Description

ExoFectin® sRNA-into-Exosome Kit  (Electro) is a proprietary formation designed for the delivery of small RNA including miRNA and siRNA into exosome. This kit provides the following advantages:

  • high loading efficiency
  • gentle on treated exosomes
    •  
  • easy to use

Shipping / Storage

 Ship and store at 4℃. (Do not freeze.)

Shelf Life

 6 months

Manual (protocol)

   
   ExoFectin® sRNA-into-Exosome Kit  (Electro)

Citation
Citation 1:
MARTINEZ N., SAITTA S., PATACQ C.   Utilisation d’exosomes comme bithérapie contre le cancer du colon .  Projet de recherche 2013-2014.

Components
ComponentsAmountStorage
ExoFectin Solution A 700 ul 4℃
ExoFectin Solution B 400 ul 4℃
Electroporation cuvettes 10 Room Temperature
Sterile transfer pipettes 10 Room Temperature

Remarks
  • The efficiency of electroporation transfection depends on the quality of the exosomes.  We do not suggest to start with impure exosome samples prepared by PEG method.
  • The efficiency of electroporation also depends on the optimal concentration of the exosomes and small RNA.   Different RNA and exosome from different source may have different optimal concentration.  We suggest customer to test different combinations in the first experiment.